Bacillus cycloheptanicus Broth

[所属分类:培养基配方] [发布时间:2019-11-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus cycloheptanicus Broth
Composition per 1001.0mL:
Solution A...............................................................1.0L
Solution B (Trace elements solution SL-6) ..............................1.0mL
pH 4.0 ± 0.2 at 25°C
Solution A:
Composition per liter:
Yeast extract.............................................................5.0g
Glucose...................................................................5.0g
KH2PO4 ...................................................................3.0g
MgSO4 ·7H2O..............................................................0.5g
CaCl2 ·2H2O ............................................................0.25g
(NH4)2SO4 ................................................................0.2g
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.0. Au-
toclave for 15 min at 15 psi pressure–121°C.
Solution B (Trace Elements Solution SL-6):
Composition per liter:
MnCl2 ·4H2O ...............................................................0.5g
H3BO3 ......................................................................0.3g
CoCl2 ·6H2O................................................................0.2g
ZnSO4 ·7H2O................................................................0.1g
Na2MoO4 ·2H2O.............................................................0.03g
NiCl2 ·6H2O...............................................................0.02g
CuCl2 ·2H2O...............................................................0.01g
Preparation of Solution B (Trace Elements Solution SL-6):
Add components to distilled/deionized water and bring volume to
1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Aseptically combine 1.0L of sterile solu-
tion A and 1.0mL of sterile solution B. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use:  For the cultivation of Bacillus cycloheptanicus, Alicyclobacillus
acidoterrestris, and Alicyclobacillus cycloheptanicus.