Bacillus Medium

[所属分类:培养基配方] [发布时间:2019-11-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bacillus Medium
Composition per liter:
(NH4)2HPO4 ..................................................................................1.0g
MgSO4 ·7H2O................................................................................0.2g
KCl................................................................................................0.2g
Yeast extract..................................................................................0.2g
Glucose solution ......................................................................50.0mL
Bromcresol Purple solution .....................................................15.0mL
pH 7.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose .......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Bromcresol Purple Solution:
Composition per 20.0mL:
Bromcresol Purple ......................................................................0.32g
Ethanol (95% solution)............................................................20.0mL
Preparation of Bromcresol Purple Solution: Add Bromcresol
Purple to 20.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Distribute 9.5mL volumes
into test tubes that contain an inverted Durham tube. Autoclave for 20
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 0.5mL of
sterile glucose to each tube. Mix thoroughly.
Use: For cultivation and differentiation of Bacillus species based on
acid and gas production from glucose.