Bacillus pasteurii Agar

[所属分类:培养基配方] [发布时间:2019-11-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bacillus pasteurii Agar
Composition per liter:
Agar ............................................................................................15.0g
Peptone..........................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................4.0g
Beef extract...................................................................................1.0g
Urea solution............................................................................50.0mL
pH 8.0 ± 0.2 at 25°C
Urea Solution:
Composition per 100.0mL:
Urea.............................................................................................20.0g
Preparation of Urea Solution:  Add urea to distilled/deionized wa-
ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Warm to 50°–55°C.
Preparation of Medium: Add components, except urea solution, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile
urea solution. Mix thoroughly. Pour into sterile Petri dishes or distrib-
ute into sterile tubes.
Use:  For the cultivation and maintenance of Bacillus pasteurii.