Bacillus pasteurii NH 4 YE Medium (Ammonium Yeast Extract Medium)

[所属分类:培养基配方] [发布时间:2019-11-28] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bacillus pasteurii NH 4 YE Medium
(Ammonium Yeast Extract Medium)
Composition per liter:
Yeast extract................................................................................20.0g
Agar ............................................................................................20.0g
(NH4)2SO4 ...................................................................................10.0g
pH 9.0 ± 0.2 at 25°C
Preparation of Medium:  Add each component to a separate flask
and bring volume of each to 333.0mL with 0.13m Tris buffer, pH 9.0.
Autoclave ingredients separately for 15 min at 15 psi pressure–121°C.
No growth occurs if components are sterilized together. Cool to 50°–
55°C and aseptically combine solutions. Pour into sterile Petri dishes.
Use:  For the cultivation and maintenance of Bacillus pasteurii.