Bacillus Pullulan Salts

[所属分类:培养基配方] [发布时间:2019-11-29] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bacillus Pullulan Salts
Composition per liter:
Pullulan.........................................................................................2.5g
NaCl..............................................................................................1.0g
NH4Cl ...........................................................................................1.0g
KH2PO4 .........................................................................................0.5g
MgSO4 ·7H2O................................................................................0.5g
Yeast extract..................................................................................0.1g
CaCl2 ·2H2O.................................................................................0.05g
Trace mineral solution .............................................................10.0mL
Vitamin solution.......................................................................10.0mL
pH 6.0 ± 0.2 at 25°C
Trace Mineral Solution:
Composition per liter:
CoCl2 ·6H2O ..................................................................................0.2g
FeSO4 ·7H2O................................................................................0.13g
ZnCl2 ·2H2O...................................................................................0.1g
MnCl2 ·4H2O..................................................................................0.1g
CaCl2 ·2H2O..............................................................................20.0mg
Na2SeO3 ...................................................................................20.0mg
Na2WO4 ·2H2O.........................................................................20.0mg
NaMoO4 ·2H2O...........................................................................1.0mg
H3BO3 ........................................................................................0.5mg
CuSO4 ·5H2O..............................................................................0.4mg
KI...............................................................................................0.1mg
Preparation of Trace Mineral Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Thiamine·HCl ............................................................................5.0mg
Riboflavin ..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
Calcium pantothenate ................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Thioctic acid..............................................................................5.0mg
Biotin.........................................................................................2.0mg
Folic acid ...................................................................................2.0mg
Cyanocobalamin........................................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except vitamin solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 6.0. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically
add sterile vitamin solution. Mix thoroughly. Aseptically distribute
into sterile tubes or flasks.
Use: For the cultivation and maintenance of Bacillus species that can
degrade pullulan.