Bacillus stearothermophilus Defined Broth

[所属分类:培养基配方] [发布时间:2019-12-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus stearothermophilus Defined Broth

培养基配方
Composition per 100.0mL:
Mineral salts solution.........................................10.0mL
Potassium phosphate buffer......................................5.0mL
L- Glutamate·HCl (1% solution).................................4.0mL
L- Leucine (1% solution).......................................1.64mL
L- Lysine·HCl (1% solution)....................................1.4mL
L- Serine (1% solution).........................................1.4mL
L- Aspartate (1% solution)......................................1.3mL
L- Valine (1% solution)........................................1.26mL
Biotin (0.01% solution) ........................................1.0mL
Glucose (20% solution) .........................................1.0mL
L- Isoleucine (1% solution).....................................1.0mL
L- Proline (1% solution).........................................1.0mL
Nicotinic acid (0.01% solution)..................................1.0mL
Thiamine·HCl (0.01% solution) ..................................1.0mL
L- Phenylalanine (1% solution)...................................0.86mL
L- Alanine (1% solution).........................................0.84mL
L- Threonine (1% solution).......................................0.84mL
L- Arginine·HCl (1% solution)...................................0.64mL
L- Tyrosine (1% solution)........................................0.56mL
L- Methionine (1% solution)......................................0.52mL
Glycine (1% solution).............................................0.5mL
L- Asparagine·H2O (1% solution)..................................0.5mL
L- Cystine (1% solution)..........................................0.5mL
L- Glutamine (1% solution)........................................0.5mL
L- Histidine·HCl·H20 (1% solution)..............................0.42mL
L- Tryptophan (1% solution).......................................0.3mL
CaCl2  (5% solution)...............................................0.01mL
FeCl3 ·6H2O (0.05% solution).......................................0.01mL
MnCl2 (10mm solution)...............................................0.01mL
ZnSO4 ·7H2O (5% solution)..........................................0.01mL
pH 7.3 ± 0.2 at 25°C
Mineral Salts Solution:
Composition per liter:
NaCl.................................................................10.0g
NH4Cl ...............................................................10.0g
MgSO4 ................................................................4.0g
Preparation of Mineral Salts Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Potassium Phosphate Buffer:
Composition per 500.0mL:
K2HPO4 ...................................................................125.0g
KH2PO4 ....................................................................30.0g
Preparation of Potassium Phosphate Buffer: Add components
to distilled/deionized water and bring volume to 500.0mL. Mix thor-
oughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Use: For the cultivation of Bacillus stearothermophilus in a chemi-
cally defined medium.