Bacillus thermoantarcticus Medium

[所属分类:培养基配方] [发布时间:2019-12-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacillus thermoantarcticus Medium

培养基配方
Composition per liter:
Yeast extract.................................................6.0g
NaCl..........................................................3.0g
Soil extract...............................................500.0mL
pH 5.6–5.8 at 25°C
Soil Extract:
Composition per liter:
Garden soil, air dried .....................................400.0g
Preparation of Soil Extract: Pass 400.0g of air-dried garden soil
through a coarse sieve. Add soil to 960.0mL of tap water. Mix thor-
oughly. Autoclave for 60 min at 15 psi pressure–121°C. Cool to room
temperature. Allow residue to settle. Decant supernatant solution. Fil-
ter through Whatman filter paper. Distribute into bottles in 200.0mL
volumes. Autoclave for 15 min at 15 psi pressure–121°C. Store at room
temperature until clear.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.6–5.8.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Bacillus thermoantarcticus.