Bacteroides cellulosolvens Medium

[所属分类:培养基配方] [发布时间:2019-12-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bacteroides cellulosolvens Medium

培养基配方
Composition per liter:
Cellobiose or cellulose....................................5.0g
NaHCO3 ....................................................2.0g
NH4Cl ....................................................0.68g
K2HPO4 ....................................................0.3g
L -Cysteine·HCl·H2O ....................................0.25g
Na2S·9H2O................................................0.25g
KH2PO4 ...................................................0.18g
(NH4)2SO4 ................................................0.15g
MgSO4·7H2O...............................................0.12g
CaCl2·2H2O...............................................0.06g
FeSO4·7H2O...............................................0.02g
Resazurin ................................................1.0mg
Trace elements solution .................................10.0mL
Vitamin solution.........................................10.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO4 ·7H2O.................................................3.0g
Nitrilotriacetic acid........................................1.5g
CaCl2 ·2H2O..................................................1.0g
NaCl..........................................................1.0g
MnSO4 ·2H2O..................................................0.5g
CoSO4 ·7H2O................................................0.18g
ZnSO4 ·7H2O.................................................0.18g
FeSO4 ·7H2O .................................................0.1g
NiCl2 ·6H20................................................0.025g
KAI(SO4)2 ·12H2O............................................0.02g
CuSO4 ·5H2O.................................................0.01g
H3BO3 .......................................................0.01g
Na2MoO4 ·2H2O...............................................0.01g
Na2SeO3 ·5H2O..............................................0.3mg
Preparation of Trace Elements Solution: Add nitrilotriacetic
acid to approximately 500.0mL distilled/deionized water. Dissolve by
adding KOH and adjust pH to 6.5. Add remaining components. Bring
volume to 1.0L with additional distilled/deionized water. Adjust pH to
7.0 with KOH.
Vitamin Solution:
Composition per liter:
Pyridoxine·HCl.................................................10.0mg
Calcium  DL -pantothenate........................................5.0mg
Lipoic acid .....................................................5.0mg
Nicotinic acid...................................................5.0mg
p-Aminobenzoic acid..............................................5.0mg
Riboflavin ......................................................5.0mg
Thiamine·HCl ...................................................5.0mg
Biotin ..........................................................2.0mg
Folic acid.......................................................2.0mg
Vitamin B 12 ....................................................0.1mg
Preparation of Vitamin Solution: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly.
Cellobiose Solution:
Composition per 50.0mL:
D- Cellobiose (or cellulose)......................................5.0g
Preparation of Cellobiose Solution: Add cellobiose (or cellulose) to
distilled/deionized water and bring volume to 50.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except cellobiose solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical-
ly add 50.0mL of sterile cellobiose (or cellulose) solution. Mix thor-
oughly. Aseptically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Bacteroides cellosolvens.