Bean Agar

[所属分类:培养基配方] [发布时间:2019-12-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Bean Agar

培养基配方
Composition per liter:
Dry white beans.............................................250.0g
Agar ........................................................20.0g
Preparation of Medium: Soak beans in 500.0mL of distilled/de-
ionized water for 12 hr. Autoclave for 20 min at 15 psi pressure–
121°C. Filter broth through cotton. Bring volume of filtrate to 1.0L
with distilled/deionized water. Add 20.0g of agar to the filtrate. Gently
heat and bring to boiling. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave
in tubes.
Use: For the cultivation and maintenance of Arthroderma melis and
Rhynchosporium secalis.