BHI Glucose Medium

[所属分类:培养基配方] [发布时间:2019-12-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

BHI Glucose Medium

培养基配方
Composition per liter:
Agar..........................................................12.0g
Pancreatic digest of gelatin..................................7.25g
Glucose........................................................6.5g
Brain heart, solids from infusion..............................3.0g
Peptic digest of animal tissue ................................3.0g
NaCl...........................................................2.5g
Na2HPO4 ......................................................1.25g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Actinomadura pelletieri,
Actinoplanes missouriensis, Actinoplanes philippinensis, Agromyces
ramosus, Corynebacterium minutissimum, Dermatophilus congolensis,
Intrasporangium calvum, Mycobacterium diernhoferi, Mycobacterium
species, Nocardia asteroides, Nocardia brevicatena, Nocardia calcarea,
Nocardia otitidiscaviarum, Pseudonocardia thermophila, Saccha-
ropolyspora rectivirgula, Streptococcus iniae, and Streptococcus pyo-
genes.