Bifidobacterium Medium

[所属分类:培养基配方] [发布时间:2019-12-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd　

Bifidobacterium Medium

培养基配方
Composition per liter:
Glucose........................................................20.0g
Pancreatic digest of casein....................................20.0g
Yeast extract..................................................10.0g
Peptone .......................................................10.0g
Tomato juice ................................................333.0mL
Tween™ 80......................................................2.0mL
pH 6.8 ± 0.2 at 25°C
Preparation of Medium:  Combine 333.0mL of tomato juice with
666.0mL of distilled/deionized water. Bring to boiling. Filter through
paper. Add remaining components to filtrate. Mix thoroughly. Bring
volume to 1.0L with distilled/deionized water. Distribute into tubes or
flasks. Autoclave for 30 min at 15 psi pressure–110°C.
Use:  For the cultivation of Bifidobacterium infantis.