Blood Base Agar with Horse Blood (LMG Medium 47)

[所属分类:培养基配方] [发布时间:2019-12-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Base Agar with Horse Blood
(LMG Medium 47)

培养基配方
Composition per liter:
Agar ......................................................15.0g
Lab-Lemco beef extract.....................................10.0g
Special peptones ..........................................10.0g
NaCl........................................................5.0g
Horse blood, sterile defibrinated.........................50.0mL
pH 7.1 ± 0.2 at 25°C
Source: Special peptones is available as a premixed powder from Ox-
oid Unipath.
Preparation of Medium: Add components, except horse blood, to
950.0mL distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL sterile
horse blood. Mix thoroughly. Pour into sterile Petri dishes or distribute
into sterile tubes.
Use: For the cultivation and maintenance of fastidious bacteria.