Blood Glucose Cystine Agar

[所属分类:培养基配方] [发布时间:2019-12-27] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Blood Glucose Cystine Agar

培养基配方
Composition per 100.0mL:
Nutrient agar............................................85.0mL
Glucose cystine solution.................................10.0mL
Human blood, fresh .......................................5.0mL
pH 6.8 ± 0.2 at 25°C
Nutrient Agar:
Composition per liter:
Agar......................................................15.0g
Pancreatic digest of gelatin...............................5.0g
Beef extract...............................................3.0g
Source:  Nutrient agar is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Nutrient Agar: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly. Gently heat
while stirring and bring to boiling. Distribute into tubes or flasks. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Glucose Cystine Solution:
Composition per 50.0mL:
Glucose ......................................................12.5g
L- Cystine·HCl................................................0.5g
Preparation of Glucose Cystine Solution: Add components to
distilled/deionized water and bring volume to 50.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: To 85.0mL of cooled, sterile agar solu-
tion, aseptically add 10.0mL of sterile glucose cystine solution and
5.0mL of human blood. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use: For the cultivation of Francisella tularensis.