Brain Heart Infusion Agar 0.7% (BHI Agar 0.7%) (BAM M23)

[所属分类:培养基配方] [发布时间:2020-1-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion Agar 0.7%
(BHI Agar 0.7%)
(BAM M23)

培养基配方
Composition per liter:
Beef heart infusion...........................................250.0g
Calf brain infusion...........................................200.0g
Proteose peptone...............................................10.0g
Agar ...........................................................7.0g
NaCl............................................................5.0g
Na2HPO4 ·12H2O.................................................2.5g
Glucose ........................................................2.0g
pH 5.3 ± 0.2 at 25°C
Source:  This medium without agar is available as a premixed powder
from BD Diagnostic Systems.
Preparation of Medium: Add components, except agar, to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad-
just pH to 5.3 with 1N HCl. Mix thoroughly. Add agar. Gently heat and
bring to boiling. Distribute into tubes. Autoclave for 10 min at 15 psi
pressure–121°C.
Use: For the detection of staphylococcal enterotoxin.