Brain Heart Infusion with Agar, Yeast Extract, Sucrose, Horse Serum, and Penicillin

[所属分类:培养基配方] [发布时间:2020-1-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with Agar, Yeast Extract,
Sucrose, Horse Serum, and Penicillin

培养基配方
Composition per liter:
Sucrose.................................................100.0g
Pancreatic digest of gelatin............................ 14.5g
Agar.....................................................12.0g
Brain heart, solids from infusion.........................6.0g
Peptic digest of animal tissue ...........................6.0g
Yeast extract.............................................5.0g
NaCl......................................................5.0g
Glucose...................................................3.0g
Na2HPO4 ..................................................2.5g
Horse serum............................................100.0mL
Penicillin solution.....................................10.0mL
pH 7.4 ± 0.2 at 25°C
Penicillin Solution:
Composition per 10.0mL:
Penicillin.............................................100,000U
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except penicillin solu-
tion and horse serum, to distilled/deionized water and bring volume to
890.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile penicillin solution and horse serum. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of fastidious fungi.