Brain Heart Infusion with PABA and Agar (Brain Heart Infusion with p-Aminobenzoic Acid and Agar)

[所属分类:培养基配方] [发布时间:2020-1-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brain Heart Infusion with PABA and Agar
(Brain Heart Infusion with
p-Aminobenzoic Acid and Agar)

培养基配方
Composition per liter:
Pancreatic digest of gelatin................................14.5g
Brain heart, solids from infusion ...........................6.0g
Peptic digest of animal tissue...............................6.0g
NaCl.........................................................5.0g
Glucose .....................................................3.0g
Na2HPO4 .....................................................2.5g
Agar ........................................................1.0g
p-Aminobenzoic acid.........................................0.05g
pH 7.4 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. The addition of 1.0g
of agar to the medium enhances the growth of anaerobic and mi-
croaerophilic microorganisms. Heat with frequent agitation and boil
for 1 min to dissolve. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the detection of microorganisms in the blood of patients who
have received sulfonamide therapy.