Brevibacterium Medium (ATCC Medium 677)

[所属分类:培养基配方] [发布时间:2020-1-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brevibacterium Medium
(ATCC Medium 677)

培养基配方
Composition per liter:
Agar .........................................................30.0g
KH2PO4 ........................................................2.0g
Na2HPO4 .......................................................2.0g
(NH4)2SO4 .....................................................2.0g
Yeast extract..................................................2.0g
Tween ™ 60.....................................................2.0g
MgSO4 ·7H2O...................................................0.2g
FeSO4· 7H2O...................................................0.1g
MnSO4 ........................................................0.01g
n-Hexadecane.................................................50.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Blend for 30 min in
a blender to disperse the n-hexadecane. Distribute into tubes or flasks.
Autoclave for 20 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Brevibacterium alkano-
philum and other microorganisms that can utilize hexadecane as a car-
bon source.