Brevundimonas Agar (LMG Medium 221)

[所属分类:培养基配方] [发布时间:2020-1-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brevundimonas Agar
(LMG Medium 221)

培养基配方
Composition per liter:
Agar..........................................................15.0g
Yeast extract.................................................10.0g
Peptone .......................................................2.0g
MgSO4 ·7H2O...................................................0.2g
CaCl2 .........................................................0.1g
Riboflavin solution...........................................5.0mL
Riboflavin Solution:
Composition per 10.0mL:
Riboflavin....................................................2.0mg
Preparation of Riboflavin Solution: Add riboflavin to 10.0mL of
distilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except riboflavin solu-
tion , to distilled/deionized water and bring volume to 995.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 5.0mL ster-
ile riboflavin solution. Mix thoroughly. Pour into sterile Petri dishes or
distribute into sterile tubes.
Use: For the cultivation of Brevundimonas spp. and Caulobacter
henricii.