Brewer Thioglycollate Medium

[所属分类:培养基配方] [发布时间:2020-1-14] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brewer Thioglycollate Medium

培养基配方
Composition per liter:
Beef, infusion from......................................500.0g
Proteose peptone..........................................10.0g
NaCl.......................................................5.0g
Glucose ...................................................5.0g
K2HPO4 ....................................................2.0g
Sodium thioglycolate.......................................0.5g
Agar.......................................................0.5g
Methylene Blue............................................2.0mg
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat to boil-
ing. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pres-
sure–121°C.
Use: For the cultivation and maintenance of anaerobic and microaero-
philic microorganisms. For testing the sterility of biological products
and materials.