Brilliance™ Enterobacter sakazakii Agar (DFI Agar) (Druggan, Forsythe and Iverson Agar)

[所属分类:培养基配方] [发布时间:2020-1-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliance™ Enterobacter sakazakii Agar
(DFI Agar)
(Druggan, Forsythe and Iverson Agar)

培养基配方
Composition per liter:
Agar........................................................15.0g
Tryptone....................................................15.0g
Soya peptone.................................................5.0g
NaCl.........................................................5.0g
Ferric ammonium citrate......................................1.0g
Sodium desoxycholate.........................................1.0g
Sodium thiosulphate .........................................1.0g
Chromogen ...................................................0.1g
pH 7.3 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the differentiation and enumeration of Enterobacter saka-
zakii from infant formula and other food samples. A chromogenic
medium for the isolation and differentiation of Enterobacter sakazakii
(now Cronobacter sakazakii) from food and dairy samples, according
to the formulation by Druggan, Forsythe, and Iverson