Brilliant Green Lactose Bile Broth (BAM M25)

[所属分类:培养基配方] [发布时间:2020-3-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brilliant Green Lactose Bile Broth
(BAM M25）

培养基配方
Composition per liter:
Oxgall, dehydrated..............................................20.0g
Lactose.........................................................10.0g
Pancreatic digest of gelatin....................................10.0g
Brilliant Green ..............................................0.0133g
pH 7.2 ± 0.1 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems and Oxoid Unipath.
Preparation of Medium:  Add lactose and pancreatic digest of gel-
atin to distilled/deionized water and bring volume to 500.0mL. Mix
thoroughly. Add 20.0g oxgall dissolved in 200.0mL distilled/deionized
water. The pH of this solution should be 7.0–7.5. Mix thoroughly.
Bring volume to 975.0mL with distilled/deionized water. Adjust pH to
7.4. Add 13.3mL of 0.1% aqueous Brilliant Green in distilled/deion-
ized water. Adjust volume to 1.0L with distilled/deionized water. Dis-
tribute into tubes containing inverted Durham tubes, in 10.0mL
amounts for testing 1.0mL or less of sample. Make sure that the fluid
level covers the inverted vials. Autoclave for 15 min at 15 psi pressure–
121°C. After sterilization, cool the broth rapidly. Medium is sensitive
to light.The final pH should be 7.2 ± 0.1 at 25°C.
Use: For the detection of coliform microorganisms in foods, dairy
products, water, and wastewater as well as in other materials of sani-
tary importance. Turbidity in the broth and gas in the Durham tube are
positive indications of Escherichia coli.