BROLACIN MUG Agar (Bromothymol Blue Lactose Cystine MUG Agar) (C.L.E.D. MUG Agar)

[所属分类:培养基配方] [发布时间:2020-3-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BROLACIN MUG Agar
(Bromothymol Blue Lactose Cystine MUG Agar)
(C.L.E.D. MUG Agar)

培养基配方

Composition per liter:
Agar...........................................................12.0g
Lactose........................................................10.0g
Universal peptone...............................................4.0g
Casein peptone..................................................4.0g
Meat extract ...................................................3.0g
L -cystine....................................................0.128g
4-Methylumbelliferyl-β- D -glucuronide.........................0.1g
Bromthymol Blue ...............................................0.02g
pH 7.3 ± 0.2 at 37°C
Source: This medium is available from Fluka, Sigma-Aldrich.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Pour into sterile Petri dishes.
Use : For the enumeration, isolation, and identification of microorgan-
isms in urine. Growth of all urinary microorganisms is favored. Lac-
tose catabolism produces a color change of Bromothymol Blue to yel-
low. Alkalization gives a color change to deep-blue. β- D -Glucoroni-
dase, which is produced by E. coli, cleaves 4-methylumbelliferyl-β- D -
glucuronide to 4-methylumbelliferone and glucuronide. The fluorogen
4-methylumbelliferone can be detected under a long wavelength UV
lamp, permitting differentiation of E. coli colonies.