Brucella FBP Broth

[所属分类:培养基配方] [发布时间:2020-3-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella FBP Broth

培养基配方
Composition per liter:
Pancreatic digest of casein..............................10.0g
Peptic digest of animal tissue ..........................10.0g
NaCl.....................................................5.0g
Yeast extract............................................2.0g
Glucose..................................................1.0g
NaHSO3 ..................................................0.1g
FBP solution...........................................30.0mL
pH 7.0 ± 0.2 at 25°C
FBP Solution:
Composition per 30.0mL:
FeSO4 ...........................................................0.25g
Sodium metabisulfite, anhydrous..................................0.25g
Sodium pyruvate, anhydrous.......................................0.25g
Preparation of FBP Solution: Add components to distilled/deion-
ized water and bring volume to 30.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except FBP solution, to
distilled/deionized water and bring volume to 970.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 30.0mL of sterile
FBP solution. Mix thoroughly. Aseptically distribute into sterile tubes.
Use: For the cultivation of Brucella species.