Brucella HiVeg Agar Base, Modified with Blood and Selective Supplement

[所属分类:培养基配方] [发布时间:2020-3-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella HiVeg Agar Base, Modified
with Blood and Selective Supplement

培养基配方
Composition per liter:
Agar .....................................................15.0g
Plant hydrolysate.........................................15.0g
Plant peptone..............................................5.0g
NaCl.......................................................5.0g
Yeast extract..............................................2.0g
Glucose ...................................................1.0g
Sodium citrate.............................................1.0g
NaHSO3 ....................................................0.1g
Horse blood, defibrinated ..............................100.0mL
Selective supplement.....................................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without horse blood or selective supplement,
is available as a premixed powder from HiMedia.
Selective Supplement:
Composition per 10.0mL:
Cycloheximide.................................................0.1g
Vancomycin..................................................20.0mg
Nalidixic acid...............................................5.0mg
Nystatin...............................................1,000,000 U
Bacitracin...............................................250,000 U
Polymyxin B sulfate ......................................50,000 U
Preparation of Selective Supplement: Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except blood and se-
lective supplement, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Heat gently with frequent mixing. Boil for
1 min. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Add 100.0mL of sterile defibrinated horse blood and 10.0mL
sterile selective supplement. Pour into sterile Petri dishes.
Use: For the cultivation and maintenance of Brucella species. For the
isolation and cultivation of nonfastidious and fastidious microorgan-
isms from a variety of clinical and nonclinical specimens.