Brucella Semisolid Medium with Nitrate

[所属分类:培养基配方] [发布时间:2020-3-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Brucella Semisolid Medium with Nitrate

培养基配方
Composition per liter:
Peptamin ....................................................10.0g
Pancreatic digest of casein..................................10.0g
Glycine......................................................10.0g
KNO3 ........................................................10.0g
NaCl..........................................................5.0g
Yeast extract.................................................2.0g
Agar .........................................................1.8g
Glucose ......................................................1.0g
NaHSO3 .......................................................0.1g
Sodium citrate................................................0.1g
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the cultivation and differentiation of Campylobacter species
based on nitrate reduction.