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培养基配方
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B.T.B. Lactose Agar, Modified (Lactose Blue HiVeg Agar)

[所属分类:培养基配方] [发布时间:2020-3-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

B.T.B. Lactose Agar, Modified
(Lactose Blue HiVeg Agar)

培养基配方
Composition per liter:
Lactose...............................................................15.5g
Agar .................................................................13.0g
NaCl..................................................................5.0g
Casein enzymatic hydrolysate .........................................3.5g
Peptone...............................................................3.5g
Bromthymol Blue .....................................................0.04g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Heat gently with fre-
quent mixing. Bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
if desired.
Use: For the isolation and cultivation of pathogenic staphylococci.

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