Buffered S & H Agar

[所属分类:培养基配方] [发布时间:2020-3-19] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Buffered S & H Agar

培养基配方
Composition per liter:
Agar................................................................15.0g
Peptone .............................................................5.0g
Yeast extract........................................................5.0g
Na2HPO4 .............................................................2.7g
Citric acid·H2O ...................................................1.15g
Glucose solution...................................................40.0mL
pH 5.0 ± 0.2 at 25°C
Glucose Solution:
Composition per 50.0mL:
Glucose.............................................................25.0g
Preparation of Glucose Solution: Add 25.0g of glucose to 50.0mL
of distilled/deionized water. Mix thoroughly and gently heat to dissolve.
Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 960.0mL. Mix
thoroughly. Gently heat to boiling. Adjust pH to 5.0 with HCl. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 40.0mL of sterile glucose solution to sterile basal medium. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Acetobacter xylinum.