Butanediol Medium

[所属分类:培养基配方] [发布时间:2020-3-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Butanediol Medium

培养基配方
Composition per liter:
NaH2PO4 ·H2O....................................................2.1g
1,4-Butanediol ..................................................1.0g
NaCl.............................................................1.0g
NH4Cl ...........................................................1.0g
CaCl2 ·2H2O ....................................................0.5g
MgSO4 ·7H2O.....................................................0.5g
K2HPO4 ..........................................................0.3g
Yeast extract....................................................0.2g
Modified Wolfe’s mineral solution.............................10.0mL
pH 7.0 ± 0.2 at 25°C
Modified Wolfe’s Mineral Solution:
Composition per liter:
MgSO4 ·7H2O....................................................3.0g
Nitrilotriacetic acid...........................................1.5g
NaCl............................................................1.0g
MnSO4 ·H2O.....................................................0.5g
CaCl2 ..........................................................0.1g
CoCl2 ·6H2O....................................................0.1g
FeSO4 ·7H2O....................................................0.1g
ZnSO4 ·7H2O....................................................0.1g
AlK(SO4)2 ·12H2O..............................................0.01g
CuSO4 ·5H2O...................................................0.01g
H3BO3 .........................................................0.01g
Na2MoO4 ·2H2O.................................................0.01g
Na2SeO3 .......................................................0.01g
NaWO4 ·2H2O ..................................................0.01g
NiC12 ·6H2O...................................................0.01g
Preparation of Modified Wolfe’s Mineral Solution: Add
nitrilotriacetic acid to 500.0mL of distilled/deionized water. Adjust pH
to 6.5 with KOH. Add remaining components one at a time. Add dis-
tilled/deionized water to 1.0L. Adjust pH to 6.8.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Pseudomonas putida.