BYE Agar

[所属分类:培养基配方] [发布时间:2020-3-24] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

BYE Agar

培养基配方
Composition per liter:
Pancreatic digest of casein.....................................16.0g
Agar............................................................13.5g
Brain heart, solids from infusion................................8.0g
Peptic digest of animal tissue ..................................5.0g
NaCl.............................................................5.0g
Glucose..........................................................2.0g
Na2HPO4 .........................................................2.5g
Yeast extract....................................................2.0g
Blood, human or animal, sterile...............................150.0mL
pH 7.8–8.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except blood, to dis-
tilled/deionized water and bring volume to 850.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 150.0mL of sterile blood. Outdated, citrated, or hepa-
rinized blood (blood from a blood bank is acceptable). Pour into sterile
Petri dishes.

Use:  For the isolation and cultivation of Mycoplasma species and  L -
forms of bacteria. For the detection of Mycoplasma species in tissue cul-
ture and cell lines.