CAE Agar Base with Triphenyltetrazolium Chloride (Citrate Azide Enterococcus HiVeg Agar Base)

[所属分类:培养基配方] [发布时间:2020-3-25] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CAE Agar Base with Triphenyltetrazolium Chloride
(Citrate Azide Enterococcus HiVeg Agar Base)

培养基配方
Composition per liter:
Agar............................................................15.0g
Casein enzymatic hydrolysate ...................................15.0g
Sodium citrate..................................................15.0g
KH2PO4 ..........................................................5.0g
Yeast extract....................................................5.0g
Na2CO3 ..........................................................2.0g
Polysorbate 80 ..................................................1.0g
NaN3 ............................................................0.4g
2,3,5-Triphenyltetrazolium chloride solution..................10.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium, without triphenyltetrazolium chloride solution,
is available as a premixed powder from HiMedia.
Caution: Sodium azide is toxic. Azides also react with metals and
disposal must be highly diluted.
2,3,5-Triphenyltetrazolium Chloride Solution:
Composition per 10.0mL:
2,3,5-Triphenyltetrazolium chloride..............................0.1g
Preparation of 2,3,5-Triphenyltetrazolium Chloride Solu-
tion: Add 2,3,5-triphenyltetrazolium chloride to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except 2,3,5-triphe-
nyltetrazolium chloride solution, to distilled/deionized water and bring
volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 10 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 10.0mL 2,3,5-triphenyltetrazolium chloride solution.
Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the isolation, cultivation, and enumeration of entercocci in
water, sewage, and feces by the membrane filter method. For the direct
plating of specimens for the detection and enumeration of fecal strep-
tococci.