Caldivirga Medium (DSMZ Medium 883)

[所属分类:培养基配方] [发布时间:2020-3-26] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Caldivirga Medium
(DSMZ Medium 883)

培养基配方
Composition per liter:
(NH4)2SO4 ..................................................1.3g
Sulfur, powdered...........................................10.0g
Na2S·9H2O.................................................0.5g
KH2PO4 ....................................................0.28g
MgSO4 ·7H2O...............................................0.25g
CaCl2 ·2H2O ..............................................0.07g
FeCl3 ·6H2O...............................................0.02g
Na2B4O7 ·10H2O............................................4.5mg
MnCl2 ·4H2O ..............................................1.8mg
Resazurin .................................................0.5mg
ZnSO4 ·7H2O..............................................0.22mg
CuCl2 ·2H2O..............................................0.05mg
Na2MoO4 ·2H2O............................................0.03mg
VOSO4 ·2H2O..............................................0.03mg
CoSO4 ....................................................0.01mg
Na2S·9H2O solution........................................7.5mL
Yeast extract solution.....................................5.0mL
Na3 -citrate·2H2O.........................................3.0mL
Vitamin solution...........................................1.0mL
pH 4.0± 0.2 at 25°C
Na2S·9H2O Solution :
Composition per 10.0mL:
Na2S·9H2O..................................................0.3g
Preparation of Na2S·9H2O Solution: Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave under 100% N2 for 15 min at 15 psi pressure–121°C. Cool
to room temperature.
Yeast Extract Solution:
Composition per 10.0mL:
Yeast extract................................................1.0g
Preparation of Yeast Extract Solution: Add yeast extract to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave under 100% N2 for 15 min at 15 psi
pressure–121°C. Cool to room temperature.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl................................................10.0mg
Thiamine-HCl·2H2O.............................................5.0mg
Riboflavin ....................................................5.0mg
Nicotinic acid.................................................5.0mg
D -Ca-pantothenate.............................................5.0mg
p-Aminobenzoic acid............................................5.0mg
Lipoic acid ...................................................5.0mg
Biotin ........................................................2.0mg
Folic acid.....................................................2.0mg
Vitamin B 12 ..................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 80% H2 + 20% CO2 . Filter sterilize.
Preparation of Medium: Add components, except sulfur, vitamin
solution, yeast extract solution, and Na2S·9H2O solution, to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH
to 3.5 with 4NH2SO4 . Sparge with 100% N2 for 30 min. Distribute into
anaerobe tubes or bottles containing sulfur, 50.0mg sulfur per 5.0mL
medium. Autoclave for 20 min at 105°C. Cool to 25°C. Before use in-
ject for every 10.0mL medium, 0.05mL sterile vitamin solution,
0.05mL sterile yeast extract, and 0.075mL sterile Na2S·9H2O solution.
Mix thoroughly. Final pH of the completed medium is around 4.0. Af-
ter inoculation, pressurize the tubes to 100 kPa overpressure with 80%
H2 + 20% CO2 gas mixture.
Use:  For the cultivation of Caldivirga maquilingensis.