Campylobacter Enrichment Broth (FDA Medium M29)

[所属分类:培养基配方] [发布时间:2020-3-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Enrichment Broth
(FDA Medium M29)

培养基配方
Composition per 1020.0mL:
Basal medium..............................................950.0mL
Horse blood, lysed.........................................50.0mL
FBP solution................................................4.0mL
Cefoperazone solution.......................................4.0mL
Trimethoprim lactate solution...............................4.0mL
Rifampicin solution ........................................4.0mL
Cycloheximide solution......................................4.0mL
pH 7.5 ± 0.2 at 25°C
Basal Medium:
Composition per 950.0mL:
Beef extract..................................................10.0g
Peptone ......................................................10.0g
Yeast extract..................................................6.0g
NaCl...........................................................5.0g
Preparation of Basal Medium:  Add components to distilled/de-
ionized water and bring volume to 950.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
FBP Solution:
Composition per 100.0mL:
FeSO4 ·7H2O....................................................6.25g
Na2S2O5 ........................................................6.25g
Sodium pyruvate.................................................6.25g
Preparation of FBP Solution:  Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Cefoperazone Solution:
Composition per 10.0mL:
Cefoperazone...................................................0.037g
Preparation of Cefoperazone Solution: Add cefoperazone to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Trimethoprim Lactate Solution:
Composition per 10.0mL:
Trimethoprim lactate...........................................0.031g
Preparation of Trimethoprim Lactate Solution: Add
trimethoprim lactate to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Rifampicin Solution:
Composition per 100.0mL:
Rifampicin .......................................................0.25g
Ethanol, absolute................................................50.0mL
Preparation of Rifampicin Solution: Add rifampicin to 50.0mL
of ethanol. Mix thoroughly. Bring volume to 100.0mL with distilled/
deionized water. Filter sterilize.
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide...................................................0.025g
Preparation of Cycloheximide Solution: Add cycloheximide to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: To 950.0mL of cooled sterile basal medi-
um, aseptically add 50.0mL of lysed (fresh, frozen, and thawed) horse
blood, 4.0mL of sterile FBP solution, 4.0mL of sterile cefoperazone so-
lution, 4.0mL of sterile trimethoprim lactate solution, 4.0mL of sterile
rifampicin solution, and 4.0mL of sterile cycloheximide solution. Mix
thoroughly. Aseptically distribute into sterile screw-capped tubes or
bottles. Close caps tightly to reduce O2 absorption. Use within 2 weeks.
Use: For the selective isolation and cultivation of Campylobacter spe-
cies from dairy products.