Campylobacter Enrichment Broth (BAM M28a)

[所属分类:培养基配方] [发布时间:2020-3-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter Enrichment Broth
(BAM M28a)

培养基配方
Composition per 1016.0mL:
Peptone ....................................................10.0g
Yeast extract................................................5.0g
Lactalbumin hydrolysate .....................................5.0g
NaCl.........................................................5.0g
α-Ketoglutamic acid.........................................1.0g
Sodium pyruvate..............................................0.5g
Na2CO3 ......................................................0.6g
Na2S2O5 .....................................................0.5g
Hemin ......................................................0.01g
Cefoperazone solution.......................................4.0mL
Trimethoprim lactate solution...............................4.0mL
Vancomycin solution ........................................4.0mL
Cycloheximide or amphotericin B solution....................4.0mL
pH 7.4 ± 0.2 at 25°C
Cefoperazone Solution:
Composition per 10.0mL:
Cefoperazone................................................0.05g
Preparation of Cefoperazone Solution: Add cefoperazone to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize. Can be stored for 5 days at 4°C, 14 days at –20°C,
and 5 months at –70°C.
Trimethoprim Solution:
Composition per 10.0mL:
Trimethoprim lactate..........................................0.066g
Preparation of Trimethoprim Solution: Add trimethoprim lac-
tate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize. Alternately add 0.05g trimethoprim hydro-
chloride to 3.0mL 0.05N HCl. Heat to 50°C. Stirr until dissolved. Add
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Filter sterilize. Can be stored for 1 year at 4°C.
Vancomycin Solution:
Composition per 10.0mL:
Vancomycin.......................................................0.05g
Preparation of Vancomycin Solution: Add vancomycin to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize. Can be stored for 2 months at 4°C.
Cycloheximide Solution:
Composition per 10.0mL:
Cycloheximide..................................................0.025g
Ethanol.........................................................2.0mL
Preparation of Cycloheximide Solution: Add cycloheximide to
2.0mL ethanol to dissolve. Mix thoroughly. Add distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Can be stored for 1 year at 4°C.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Amphotericin B Solution:
Composition per 10.0mL:
Amphotericin B..................................................0.005g
Preparation of Amphotericin B Solution: Add Amphotericin B
to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize. Can be stored for 1 year at –20°C.
Preparation of Medium: Add components, except antimicrobic
solutions, to distilled/deionized water and bring volume to 1.0L. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Aseptically add 4.0mL of sterile cefoperazone solution, 4.0mL
of sterile trimethoprim lactate solution, 4.0mL of sterile vancomycin
solution, and either 4.0mL of sterile cycloheximide solution or 4.0mL
of sterile amphotericin B solution. Mix thoroughly. Aseptically distrib-
ute into sterile screw-capped tubes or bottles. Close caps tightly to re-
duce O 2 absorption. Use within 2 weeks.
Use: For the selective isolation and cultivation of Campylobacter spe-
cies. For the pre-enrichment of Campylobacter spp. in food samples.
This medium aids resuscitation of sublethally injured cells and over-
comes the damaging effects of food processing.