Campylobacter mucosalis Medium

[所属分类:培养基配方] [发布时间:2020-4-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Campylobacter mucosalis Medium

培养基配方
Composition per liter:
Agar ........................................................15.0g
Beef extract.................................................10.0g
Special peptone..............................................10.0g
NaCl..........................................................5.0g
Sodium fumarate...............................................3.0g
Sodium formate................................................2.0g
Horse blood, defibrinated ..................................50.0mL
pH 7.2 ± 0.2 at 25°C
Source: Special peptone (L72) is available from Oxoid Unipath.
Preparation of Medium:  Add components, except horse blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Heat with frequent agitation and boil for 1 min to completely dis-
solve. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 50.0mL of sterile, defibrinated horse blood. Mix
thoroughly and pour into sterile Petri dishes.
Use:  For the cultivation and maintenance of Campylobacter mucosa-
lis.