Cantharellus Agar

[所属分类:培养基配方] [发布时间:2020-4-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cantharellus Agar

培养基配方
Composition per liter:
Agar ......................................................12.0g
Glucose ....................................................4.0g
Sodium succinate...........................................1.35g
Kl ........................................................1.21g
Na2MoO4 ...................................................1.21g
MnSO4 ·H2O...............................................0.845g
NH4Cl .....................................................0.58g
KH2PO4 .....................................................0.2g
CuSO4 ·5H2O..............................................0.125g
CoCl2 ·6H2O...............................................0.12g
MgSO4 ·H2O.................................................0.1g
CaCl2 ·H2O...............................................26.5mg
NaCl......................................................20.0mg
m-Inositol................................................10.0mg
EDTA.......................................................9.3mg
FeSO4 ·7H2O..............................................6.95mg
ZnSO4 ·7H2O..............................................1.44mg
H3BO4 ....................................................0.31mg
Calcium  DL -pantothenate..................................0.1mg
Nicotinic acid.............................................0.1mg
p-Aminobenzoic acid........................................0.1mg
Pyrdoxine HCl..............................................0.1mg
Riboflavin.................................................0.1mg
Thiamine HCl...............................................0.1mg
Biotin...................................................0.025mg
Tomato roots............................................variable
Preparation of Medium: Add components, except tomato root, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes. Add onto the agar a piece of fresh tomato root near the
inoculum.
Use:  For the cultivation and maintenance of Cantharellus cibarius.