Carboxydobacterium Medium

[所属分类:培养基配方] [发布时间:2020-4-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Carboxydobacterium Medium

培养基配方
Composition per liter:
Na2HPO4 ·12H2O............................................9.0g
KH2PO4 ....................................................1.5g
NH4Cl .....................................................1.5g
MgSO4 ·7H2O...............................................0.2g
CaCl2 ·2H20.............................................20.0mg
Ferric ammonium citrate...................................1.2mg
TS2 trace elements solution...............................1.0mL
TS2 Trace Elements Solution:
Composition per liter:
Na2MoO4 ·2H2O ............................................0.9g
H3BO3 .....................................................0.3g
CoCl2 ·6H2O ..............................................0.2g
ZnSO4 ·7H2O...............................................0.1g
MnCl2 ·4H2O.............................................30.0mg
Na2SeO3 .................................................20.0mg
NiCl2 ·6H2O.............................................20.0mg
CuCl2 ·2H20 ............................................10.0mg
Preparation of TS2 Trace Elements Solution:  Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Caution:  Carbon monoxide (CO) is a toxic gas.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. After inocu-
lation, incubate in an atmosphere of 50% CO + 50% air.
Use: For the autotrophic cultivation of Oligotropha carboxidovorans.