Casman HiVeg Broth Base with Blood

[所属分类:培养基配方] [发布时间:2020-4-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CASO MUG Agar

培养基配方
Composition per liter:
Casein peptone............................................16.0g
Agar .....................................................13.0g
NaCl.......................................................6.0g
Soy peptone................................................5.0g
Tryptophan.................................................1.0g
4-Methylumbelliferyl-β- D -glucuronide...................0.07g
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from Fluka, Sigma-Aldrich.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Pour into sterile Petri dishes.
Use: This universal medium without indicator or inhibitor is intended
for a broad range of application, including enumeration and cultivation
of a wide variety of microorganisms. It is also suitable for the cultiva-
tion of more fastidious microorganisms. β- D -glucoronidase, which is
produced by E. coli, cleaves 4-methylumbelliferyl-β- D -glucuronide to
4-methylumbelliferone and glucuronide. The fluorogen 4-methylum-
belliferone can be detected under a long wavelength UV lamp. A pos-
itive indole reaction provides confirmation.