Castenholz Medium

[所属分类:培养基配方] [发布时间:2020-4-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Castenholz Medium

培养基配方
Composition per liter:
Tryptone.....................................................1.0g
Yeast extract................................................1.0g
NaNO3 ....................................................689.0mg
Na2HPO4 ·2H2O............................................140.0mg
KNO3 .....................................................103.0mg
MgSO4 ·7H2O..............................................100.0mg
Nitrilotriacetic acid.....................................100.0mg
CaSO4 ·2H2O...............................................60.0mg
NaCl........................................................8.0mg
MnSO4 ·H2O.................................................2.2mg
H3BO3 ......................................................0.5mg
ZnSO4 ·7H2O................................................0.5mg
FeCl3 ·6H2O...............................................0.47mg
CoCl2 ·6H2O .............................................46.0μg
CuSO4 ·5H2O.............................................25.04μg
Na2MoO4 ·2H2O ...........................................25.0μg
pH 8.2 ± 0.2 at 25°C
Preparation of Medium: Combine components. Mix thoroughly.
Adjust pH to 8.2 with NaOH. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Thermus aquaticus.