CAT Medium (Campylobacter Blood Free Preson Agar with Cefoperazone, Amphotericin, and Teicoplanin)

[所属分类:培养基配方] [发布时间:2020-4-10] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

CAT Medium
(Campylobacter Blood Free Preson Agar
with Cefoperazone, Amphotericin, and Teicoplanin)

培养基配方
Composition per liter:
Agar.........................................................12.0g
Beef extract.................................................10.0g
Peptone .....................................................10.0g
NaCl..........................................................5.0g
Charcoal......................................................4.0g
Casein hydrolysate............................................3.0g
Sodium deoxycholate...........................................1.0g
FeSO4 .......................................................0.25g
Sodium pyruvate..............................................0.25g
Selective supplement solution ..............................10.0mL
pH 7.5 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Selective Supplement Solution:
Composition per 10.0mL:
Amphotericin...............................................10.0mg
Sodium cefoperazone.........................................8.0mg
Teicoplanin ................................................4.0mg
Preparation of Selective Supplement Solution: Add sodium
cefoperazone to distilled/deionized water and bring volume to 10.0mL.
Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution, to distilled/deionized water and bring volume to
990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add 10.0mL of sterile selective supplement solution. Mix thoroughly.
Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Campylobacter species. For the isolation of
Campylobacter spp., especially Campylobacter upsaliensis.