Celery Decoction Agar

[所属分类:培养基配方] [发布时间:2020-4-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Celery Decoction Agar

培养基配方
Composition per liter:
Potatoes, infusion from...................................200.0g
Glucose....................................................20.0g
Agar.......................................................15.0g
Potato dextrose broth.......................................4.0g
Celery decoction.........................................320.0mL
pH 5.6 ± 0.2 at 25°C
Potatoes, Infusion From:
Composition per 500.0mL:
Potatoes.................................................300.0g
Preparation of Potatoes, Infusion From:  Peel and dice potatoes.
Add 500.0mL of distilled/deionized water. Gently heat and bring to
boiling. Continue boiling for 30 min. Filter through cheesecloth.
Celery Decoction:
Composition per 500.0mL:
Celery leaves and petioles ...............................50.0g
Preparation of Celery Decoction: Coarsely cut celery into pieces.
Add 500.0mL of distilled/deionized water. Puree in a blender at high
speed for 2 min. Strain through a kitchen-type sieve. Gently heat and
bring to boiling. Continue boiling for 5 min. Strain through a very fine
sieve or muslin.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.6 with
20% H2SO4 . Gently heat and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile
Petri dishes or leave in tubes.
Use: For the cultivation of Septoria apiicola.