Cellulolytic Broth for Thermophiles

[所属分类:培养基配方] [发布时间:2020-4-13] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cellulolytic Broth for Thermophiles

培养基配方
Composition per liter:
K2HPO4 ...................................................1.65g
NH4SO4 ....................................................1.6g
Yeast extract..............................................1.0g
NaCl......................................................0.96g
L -Cysteine·HCl·H2O......................................0.5g
CaCl2 ...................................................0.096g
MgSO4 ...................................................0.096g
Resazurin (0.1% solution) ................................1.0mL
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Prepare and dispense medium anaerobi-
cally in 100% N2 . Add components to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2 with 5M
NaOH. Distribute into tubes or flasks that contain cellulose as a strip
(4.5cm × 1.0cm) of Whatman #1 filter paper. Autoclave for 15 min at
15 psi pressure–121°C.
Use:  For the cultivation of Clostridium stercorarium and other bacte-
ria that can utilize cellulose as a carbon source.