Cetrimide Agar Base with Glycerol and Nalidixic Selective Supplement

[所属分类:培养基配方] [发布时间:2020-4-15] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cetrimide Agar Base
with Glycerol and Nalidixic Selective Supplement

培养基配方
Composition per liter:
Pancreatic digest of gelatin..................................20.0g
K2SO4 ........................................................10.0g
MgCl2 .........................................................1.4g
Cetrimide......................................................0.3g
Glycerol ....................................................10.0mL
Nalidixic selective supplement................................5.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without glycerol and nalidixic acid supple-
ment, is available as a premixed powder from HiMedia.
Nalidixic Selective Supplement:
Composition per 5.0mL:
Nalidixic acid...............................................15.0mg
Preparation of Nalidixic Selective Supplement: Add nalidixic
acid to distilled/deionized water and bring volume to 50.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except nalidixic selec-
tive supplement, to distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add 5.0mL sterile nalidixic selective supplement.
Mix thoroughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the selective isolation, cultivation, and identification of
Pseudomonas aeruginosa and other Gram-negative, nonfermentative
bacteria.