Chitin Agar

[所属分类:培养基配方] [发布时间:2020-4-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chitin Agar

培养基配方
Composition per liter:
Agar........................................................15.0g
Chitin, precipitated ........................................3.0g
(NH4)2SO4 ...................................................2.0g
Na2HPO4 .....................................................1.1g
KH2PO4 ......................................................0.7g
MgSO4 ·7H2O.................................................0.2g
FeSO4 ......................................................1.0mg
MnSO4 ......................................................1.0mg
Chitin, Precipitated:
Composition  per 2.5L:
Chitin......................................................40.0g
HCl, concentrated.........................................400.0mL
Preparation of Chitin, Precipitated: Add chitin to 400.0mL of
cold concentrated HCl. Add this solution to 2.0L of distilled/deionized
water at 5°C. Filter the solution through Whatman #1 filter paper. Dia-
lyze the precipitated chitin against tap water for 12 hr. Adjust the pH to
7.0 with KOH.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of Cytophaga species, Herpeto-
siphon species, Saprospira species, and Flexithrix species.