Chlamydia Growth Medium

[所属分类:培养基配方] [发布时间:2020-4-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chlamydia Growth Medium

培养基配方
Composition per 500.0mL:
Eagle minimum essential medium
with Earle salts, 10X............................................50.0mL
Fetal calf serum.................................................50.0mL
L- Glutamine solution.............................................5.0mL
pH 7.4 ± 0.2 at 25°C
Eagle Minimum Essential Medium with Earle Salts, 10X:
Composition per liter:
NaCl...............................................................6.8g
Glucose ...........................................................1.0g
KCl................................................................0.4g
CaCl2 ·2H2O.......................................................0.2g
MgCl2 ·6H2O.......................................................0.2g
NaH2PO4 ..........................................................0.15g
L- Arginine .......................................................0.1g
L- Lysine.........................................................0.06g
L- Isoleucine.....................................................0.05g
L- Leucine........................................................0.05g
L- Threonine......................................................0.05g
L- Valine ........................................................0.05g
L- Tyrosine.......................................................0.04g
L- Phenylalanine..................................................0.03g
L- Histidine......................................................0.03g
L- Cystine .......................................................0.02g
L- Methionine.....................................................0.02g
L- Tryptophan.....................................................0.01g
i-Inositol........................................................2.0mg
Calcium pantothenate .............................................1.0mg
Choline chloride..................................................1.0mg
Folic acid........................................................1.0mg
Nicotinamide......................................................1.0mg
Pyridoxal.........................................................1.0mg
Thiamine·HCl ....................................................1.0mg
Riboflavin .......................................................0.1mg
Preparation of Eagle Minimum Essential Medium with
Earle Salts, 10X: Add components to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4 with 7.5%
Na2CO3 solution. Filter sterilize.
Glutamine Solution:
Composition per 100.0mL:
L- Glutamine......................................................2.92g
NaCl (0.85% solution)...........................................100.0mL
Preparation of Glutamine Solution: Add the glutamine to the
0.85% NaCl solution. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically combine 50.0mL of sterile Ea-
gle minimum essential medium with Earle salts, 10X, 50.0mL of fetal calf
serum, and 5.0mL of sterile glutamine solution. Bring volume to 500.0mL
with sterile distilled/deionized water. Mix thoroughly. Aseptically distrib-
ute into sterile tubes or flasks.
Use: For the cultivation of Chlamydia species.