Chlamydomonas Mutant Broth

[所属分类:培养基配方] [发布时间:2020-4-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chlamydomonas Mutant Broth

培养基配方
Composition per liter:
K2HPO4 .....................................................1.162g
Sodium acetate................................................1.0g
NaH2PO4 .....................................................0.92g
Sodium citrate................................................0.5g
NH4NO3 .......................................................0.3g
MgSO4 ·7H2O..................................................0.3g
CaCl2 .......................................................0.04g
FeCl3 ·6H2O.................................................0.01g
Trace salts solution .......................................10.0mL
pH 6.8 ± 0.2 at 25°C
Trace Salts Solution:
Composition per liter:
H3BO3 .......................................................0.15g
LiSO4 ·4H2O..................................................0.1g
MnSO4 ·4H2O.................................................0.04g
CoCl2 .......................................................0.02g
(NH4)6Mo7O24 ...............................................0.015g
CuSO4 ·5H2O................................................0.004g
Preparation of Trace Salts Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.8.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Chlamydomonas reinhardtii.