Chloramphenicol Ampicillin LB Medium

[所属分类:培养基配方] [发布时间:2020-4-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chloramphenicol Ampicillin LB Medium

培养基配方
Composition per liter:
NaCl...........................................................10.0g
Pancreatic digest of casein....................................10.0g
Yeast extract...................................................5.0g
Ampicillin solution...........................................10.0mL
Chloramphenicol solution......................................10.0mL
pH 7.0 ± 0.2 at 25°C
Ampicillin Solution:
Composition per 10.0mL:
Ampicillin....................................................40.0mg
Preparation of Ampicillin Solution: Add ampicillin to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Chloramphenicol Solution:
Composition per 10.0mL:
Chloramphenicol.................................................5.0mg
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except ampicillin solu-
tion and chloramphenicol solution, to distilled/deionized water and
bring volume to 980.0mL. Mix thoroughly. Adjust pH to 7.0. Auto-
clave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of
sterile ampicillin solution and 10.0mL of sterile chloramphenicol solu-
tion. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Escherichia coli.