Chloramphenicol L Broth Medium No. 1

[所属分类:培养基配方] [发布时间:2020-4-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chloramphenicol L Broth Medium No. 1

培养基配方
Composition per liter:
Pancreatic digest of casein....................................10.0g
NaCl............................................................5.0g
Yeast extract...................................................5.0g
Glucose ........................................................1.0g
Chloramphenicol solution......................................10.0mL
pH 7.0 ± 0.2 at 25°C
Chloramphenicol Solution:
Composition per 10.0mL:
Chloramphenicol...............................................5.0mg
Preparation of Chloramphenicol Solution: Add chlorampheni-
col to distilled/deionized water and bring volume to 10.0mL. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except chlorampheni-
col solution, to distilled/deionized water and bring volume to 990.0mL.
Mix thoroughly. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically add 10.0mL of sterile chloramphenicol solu-
tion and 10.0mL of sterile chloramphenicol solution. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Escherichia coli.