Chocolate Tellurite Agar (Tellurite Blood Agar)

[所属分类:培养基配方] [发布时间:2020-4-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Chocolate Tellurite Agar
(Tellurite Blood Agar)

培养基配方
Composition per liter:
Agar..............................................................10.0g
Casein/meat (50/50) peptone.......................................10.0g
Hemoglobin........................................................10.0g
NaCl...............................................................5.0g
K2HPO4 ............................................................4.0g
Cornstarch.........................................................1.0g
KH2PO4 ............................................................1.0g
K2TeO3 ............................................................0.1g
Bio-X enrichment ................................................10.0mL
Bio-X Enrichment:
Composition per liter:
Glucose..........................................................100.0g
L -Cysteine·HCl .................................................25.9g
L- Glutamate......................................................10.0g
L- Cystine.........................................................1.1g
Adenine............................................................1.0g
Cocarboxylase......................................................0.1g
Guanine·HCl......................................................0.03g
FeNO3 ............................................................0.02g
p-Aminobenzoic acid..............................................0.013g
Vitamin B 12 .....................................................0.01g
NAD (nicotinamide adenine dinucleotide).........................250.0mg
Thiamine·HCl ....................................................3.0mg
pH 7.2 ± 0.2 at 25°C
Preparation of Bio-X Enrichment: Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium:  Add components, except Bio-X enrich-
ment, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add filter-ster-
ilized Bio-X enrichment. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use:  For the selective isolation and cultivation of Corynebacterium
species. Corynebacterium diphtheriae appears as gray-black colonies.