Cholera Medium Base with Tellurite and Blood

[所属分类:培养基配方] [发布时间:2020-4-22] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cholera Medium Base with Tellurite and Blood

培养基配方
Composition per liter:
NaCl...........................................................20.0g
Agar ..........................................................10.0g
Peptic digest of animal tissue.................................10.0g
Beef extract...................................................10.0g
Sucrose........................................................10.0g
Na2CO3 .........................................................5.0g
Sodium lauryl sulfate...........................................0.1g
Sheep blood, defibrinated.....................................50.0mL
Tellurite solution.............................................2.0mL
pH 8.5 ± 0.2 at 25°C
Source: This medium, without tellurite or blood, is available as a pre-
mixed powder from HiMedia.
Tellurite Solution:
Composition per 10.0mL:
K2TeO3 .........................................................0.1g
Preparation of Tellurite Solution: Add K2TeO3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Do not autoclave. Cool to 70°C. Aseptically add 2.0mL of
sterile tellurite soltuion and 50.0mL of sterile defibrinated blood.
Maintain at 70°C for several minutes. Cool to 50°C. Pour into sterile
Petri dishes or leave in tubes.
Use:  For the isolation of pathogenic vibrios, especially Vibrio chol-
erae. For the selective isolation of Vibrio species from specimens
grossly contaminated with Enterobacteriaceae.