Cholera Medium TCBS

[所属分类:培养基配方] [发布时间:2020-4-23] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Cholera Medium TCBS

培养基配方
Composition per liter:
Sucrose......................................................20.0g
Agar.........................................................14.0g
Peptone .....................................................10.0g
Na2S2O3 .....................................................10.0g
Sodium citrate...............................................10.0g
NaCl.........................................................10.0g
Ox bile ......................................................8.0g
Yeast extract.................................................5.0g
Ferric citrate................................................1.0g
Bromthymol Blue..............................................0.04g
Thymol Blue..................................................0.04g
pH 8.6 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0mL. Mix thoroughly. Gently heat while
stirring and bring to boiling. Do not autoclave. Cool to 45°C. Pour into
sterile Petri dishes.
Use:  For the isolation of pathogenic vibrios, especially Vibrio chol-
erae. This medium is suitable for the growth of Vibrio cholerae, Vibrio
parahaemolyticus, and most other Vibrios. Most of the Enterobacteri-
aceae encountered in feces are totally suppressed for at least 24 hours.
Slight growth of Proteus species and Enterococcus faecalis may occur
but the colonies are easily distinguished from vibrio colonies. While
inhibiting non-vibrios, it promotes rapid growth of pathogenic vibrios
after overnight incubation at 35°C. Vibrio cholerae El Tor biotype
forms yellow colonies, Vibrio parahaemolyticus forms blue-green col-
onies, Vibrio alginolyticus forms yellow colonies, Vibrio metschnik-
ovii forms yellow colonies, Vibrio fluvialis forms yellow colonies,
Vibrio vulnificus forms blue-green colonies, Vibrio mimicus forms
blue-green colonies, Enterococcus species form yellow colonies, Pro-
teus species form yellow-green colonies, Pseudomonas species form
blue-green colonies and some strains of Aeromonas hydrophila pro-
duce yellow colonies, but Plesimonas shigelloides does not usually
grow well on this medium.